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詳細介紹
| 品牌 | 其他品牌 | 貨號 | BI |
|---|---|---|---|
| 供貨周期 | 一個月 | 應用領域 | 醫療衛生,化工,生物產業,制藥/生物制藥,綜合 |
lucernatechnologies：BI 產品編號：600-1mg
分子式：C19H14F2N4O2
分子量：368.3
(Z)-3-((1H-benzo[d]imadazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-3,5-dihydro-4H-imidazol-4-one (BI)
分子式：C19H14F2N4O2
分子量:368.3
CAS NAME/NUMBER:
Absorption and fluorescence emission spectra of BroccoliTM/BI in pH 7.4 buffer.
PRESENTATION:凍干染料
質量保證：產品由以下方式分析1H NMR 和 LC-MS，并通過 HPLC 以 >98% 的純度提供。
使用聲明：本產品僅供研究使用，不得用于任何其他目的，包括但不限于未經授權的商業用途、體外診斷用途、離體或體內治療用途或任何類型的消費或對人類或動物的應用。由于熒光團和適配體的高度特異性，我們無法預測或對 Lucerna 如何負責TM的產品將在其客戶的系統中運行。使用 Lucerna 的研究人員TM的產品應進行優化研究，以達到其預期應用的最佳結果。
Figure 1. Live-cell imaging of COS7 cells expressing β-actin mRNA transcripts tagged with 24 copies of BroccoliTM in the 3’UTR. Cells were incubated with 0.5 µg/ml Hoechst 33342 stain (blue) and 10 µM BI (green) and images were acquired using a 500 msec exposure with a GFP filter set. Mobile fluorescence puncta were observed only when Broccoli-expressing cells were treated with BI.
Figure 2. For single-molecule imaging, COS7 cells were transfected with a construct expressing mCherry-24xBroccoliTM transcripts and imaged in the presence 10 µM BI. Images of the same cells were acquired using TRITC and GFP filter sets to capture mCherry fluorescence (red) and BroccoliTM (green) signals. Mobile fluorescent puncta were detected in both the nuclei and cytosol of mCherry-expressing cells.
Figure 3. Live fluorescent mRNA puncta were imaged using COS7 cells expressing either A. the MS2-GFP system and B. the BroccoliTM-BI system. The puncta size of Broccoil-tagged mRNA is similar to the puncta size of MS-tagged mRNA. Further, the puncta intensities of mRNA labeled from both systems exhibit a single Guassian distribution, consistent with the majority of these puncta reflecting single mRNA transcripts. The scale bars, are both 5 µm.
Fluorophore-promoted RNA folding and photostability enable imaging of single Broccoli-tagged mRNAs in living mammalian cells. Li X, Kim HY, Litke JL, Wu JH, Jaffrey SR. Angew Chem Int Ed. 2020 Mar 9;59(11): 4511-4518.
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lucernatechnologies：BI 產品編號：600-1mg
分子式：C19H14F2N4O2
分子量：368.3
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